{"id":71345,"date":"2022-09-19T09:09:32","date_gmt":"2022-09-19T07:09:32","guid":{"rendered":"https:\/\/ogrod-powsin.pl\/paproc-drzewiasta-w-sterylnej-kulturze-zawiesinowej\/"},"modified":"2024-10-27T06:34:06","modified_gmt":"2024-10-27T05:34:06","slug":"tree-fern-in-sterile-suspension-culture","status":"publish","type":"post","link":"https:\/\/ogrod.pan.pl\/en\/paproc-drzewiasta-w-sterylnej-kulturze-zawiesinowej\/","title":{"rendered":"Tree fern in sterile suspension culture"},"content":{"rendered":"

Although liquid in vitro cultures are a well-established method for multiplying plant cells and a source for obtaining plants on a massive scale, as well as a pathway for producing a range of valuable secondary metabolites, in the case of ferns, this type of culture was only described in 2022 by our Garden's biotechnology team. The study included tree ferns Cyathea smithii<\/em>, which is native to New Zealand, where it grows up to 8 meters tall. It is one of the few woody ferns that can survive occasional snowfall and frosts down to about -5o<\/sup>C. A distinctive feature of this species is the maintenance of a \"skirt\" of dry leaves on the vertically erect rhizome, which protects the meristem from adverse environmental conditions.<\/p>\n

The research conducted allowed to describe the structure and growth potential of the cell suspension C. smithii<\/em> which provides a basis for further experimentation. Extacts prepared from plant tissue showed antimicrobial activity against Staphylococcus aureus, Escherichia coli<\/em> i Pseudomonas aeruginosa<\/em>, which can find practical use.<\/p>\n

Rybczynski JJ, Kazmierczak A, Dos Santos Szewczyk K, Tomaszewicz W, Miazga-Karska M, Mikula A 2022<\/strong> Biotechnology of the tree fern Cyathea smithii<\/em> (J.D. Hooker; Soft tree fern, Katote). II. Cell suspension culture: focusing on structure and physiology in the presence of 2,4-D and BAP. Cells 11, 1396. https:\/\/doi.org\/10.3390\/cells11091396<\/p>\n

<\/p>\n

Steps leading to the derivation of liquid culture Cyathea smithii<\/em>: (A) obtaining sterile in vitro culture of plants on agar medium (view of root system as viewed from the bottom of the jar); (B) root fragment with visible callus tissue used to initiate suspension cultures; (C) liquid culture of callus tissue cells; (D) suspension cells - light microscope image; (E) suspension cells - scanning electron microscope image; (F) cells C. smithii<\/em> As an object of experimentation - the visible interior of the cells.<\/p>\n

post<\/p>\n

post<\/p>\n

2022-09-19 09:09:32<\/p>","protected":false},"excerpt":{"rendered":"

Mimo \u017ce p\u0142ynne kultury in vitro s\u0105 dobrze poznan\u0105 metod\u0105 namna\u017cania kom\u00f3rek ro\u015blinnych i \u017ar\u00f3d\u0142em pozyskiwania ro\u015blin na masow\u0105 skal\u0119 oraz drog\u0105 produkowania szeregu cennych metabolit\u00f3w wt\u00f3rnych, to w przypadku paproci, ten typ kultury zosta\u0142 opisany dopiero w 2022 roku przez zesp\u00f3\u0142 biotechnolog\u00f3w naszego Ogrodu. Badaniami obj\u0119to papro\u0107 drzewiast\u0105 Cyathea smithii, kt\u00f3ra pochodzi z Nowej…<\/p>","protected":false},"author":23,"featured_media":71348,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","ngg_post_thumbnail":0,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":"","footnotes":""},"categories":[474],"tags":[],"class_list":["post-71345","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-informacje-naukowe"],"taxonomy_info":{"category":[{"value":474,"label":"Informacje naukowe"}]},"featured_image_src_large":["https:\/\/ogrod.pan.pl\/wp-content\/uploads\/2024\/10\/Slajd1-1024x576.png",1024,576,true],"author_info":{"display_name":"login_at","author_link":"https:\/\/ogrod.pan.pl\/en\/author\/login_at\/"},"comment_info":0,"category_info":[{"term_id":474,"name":"Informacje naukowe","slug":"informacje-naukowe","term_group":0,"term_taxonomy_id":474,"taxonomy":"category","description":"","parent":0,"count":129,"filter":"raw","term_order":"0","cat_ID":474,"category_count":129,"category_description":"","cat_name":"Informacje naukowe","category_nicename":"informacje-naukowe","category_parent":0}],"tag_info":false,"meta_box":{"mb_document_group":[]},"_links":{"self":[{"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/posts\/71345","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/users\/23"}],"replies":[{"embeddable":true,"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/comments?post=71345"}],"version-history":[{"count":0,"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/posts\/71345\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/media\/71348"}],"wp:attachment":[{"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/media?parent=71345"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/categories?post=71345"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ogrod.pan.pl\/en\/wp-json\/wp\/v2\/tags?post=71345"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}